《植物生理学报》 2018, 54(7): 1159-1171

植物MLO蛋白研究进展

张孝廉^1,2, 张吉顺^1,2, 雷波², 余婧², 赵德刚^1,3,*

¹贵州大学生命科学学院/农业生物工程研究院, 贵州省农业生物工程重点实验室, 山地植物资源保护与种质创新省部共建教育部重点实验室, 贵阳550025; ²贵州省烟草科学研究院, 烟草行业分子遗传重点实验室, 贵阳550081; ³贵州省农业科学院, 农业部DUS中心贵阳分中心, 贵阳550006

通信作者：赵德刚；E-mail: dgzhao@gzu.edu.cn

摘　要：

Mildew resistance locus O (MLO)蛋白是植物特有的一类蛋白家族, 其中的部分成员是白粉病菌成功侵染寄主植物的必要因子。一个或多个MLO基因的功能缺失突变可赋予植物对白粉病菌广谱且持久的抗性, mlo介导的白粉病抗性在抗病育种中发挥了重要作用。植物中的MLO蛋白可以分为7个亚家族, 除了参与植物与白粉病菌的互作外, 不同亚家族的MLO蛋白还有很多其他功能。本文对MLO蛋白的特征、分类、功能进行了归纳, 介绍了植物与白粉病抗性相关的mlo突变体特征及抗病机制, 并对MLO基因的功能、作用机理研究及其在植物抗病育种中利用应注意的问题进行了讨论。

关键词：植物; MLO蛋白; 白粉病; mlo抗性

收稿：2018-04-09   修定：2018-07-10

资助：中国烟草总公司贵州省公司科技项目(201605和201606)、国家转基因生物新品种培育重大专项(2016ZX08010003-009)和贵州省高层次创新型人才培养项目[黔科合人才(2016)4003号]。

Research progress of MLO proteins in plants

ZHANG Xiao-Lian^1,2, ZHANG Ji-Shun^1,2, LEI Bo², YU Jing², ZHAO De-Gang^1,3,*

¹Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Laboratory of Agro-bioengineering, Institute of Agro-bioengineering / College of Life Sciences, Guizhou University, Guiyang 550025, China; ²Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang 550081, China; ³Guiyang Station for DUS Testing Center of New Plant Varieties of MOA, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China

Corresponding author: ZHAO De-Gang; E-mail: dgzhao@gzu.edu.cn

Abstract:

Mildew resistance locus O (MLO) is a plant-specific protein family, some of which are essential factors for the powdery mildew successfully infection. Loss-of-function mutation of one or more MLO genes can confer the host plants durable broad-spectrum resistance to powdery mildew. mlo-based resistance has been successfully employed in plant resistance breeding. Plant MLO proteins can be divided into 7 subfamilies. In addition to the plant-powdery mildew interaction, MLOs from different subfamilies may have different functions. This review introduces the characteristic, categorization and function of MLO proteins, as well as the features and possible resistance mechanisms of mlo mutant. At last, we discuss the function mechanism of MLO proteins, the utilization of MLO genes in the plant disease resistance breeding and the problems we should pay attention to.

Key words: plant; MLO protein; powdery mildew; mlo resistance